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Addgene

pTCW022
(Plasmid #83532)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83532 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS406
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t-pFUS1J2-TKL1-CYC1t
  • Species
    S. cerevisiae (budding yeast)
  • Promoter pFUS1J2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13pUC-Fwd
  • 3′ sequencing primer M13pUC-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTCW022 was a gift from Claudia Vickers (Addgene plasmid # 83532 ; http://n2t.net/addgene:83532 ; RRID:Addgene_83532)

  • For your References section:

    Quorum-sensing linked RNA interference for dynamic metabolic pathway control in Saccharomyces cerevisiae. Williams TC, Averesch NJH, Winter G, Plan MR, Vickers CE, Nielsen LK, Kromer JO. Metab Eng. 2015 May;29:124-134. doi: 10.1016/j.ymben.2015.03.008. Epub 2015 Mar 16. 10.1016/j.ymben.2015.03.008 PubMed 25792511
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