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Addgene

pTCW010
(Plasmid #83521)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83521 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS413
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pARO9-mfalpha2
  • Species
    S. cerevisiae (budding yeast)
  • Promoter pARO9

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13pUC-Fwd
  • 3′ sequencing primer M13pUC-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTCW010 was a gift from Claudia Vickers (Addgene plasmid # 83521 ; http://n2t.net/addgene:83521 ; RRID:Addgene_83521)

  • For your References section:

    Engineered quorum sensing using pheromone-mediated cell-to-cell communication in Saccharomyces cerevisiae. Williams TC, Nielsen LK, Vickers CE. ACS Synth Biol. 2013 Mar 15;2(3):136-49. doi: 10.1021/sb300110b. Epub 2013 Jan 7. 10.1021/sb300110b PubMed 23656437
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