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pGB1-mCRT(P-domain)
(Plasmid #83519)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83519 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGB1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CALR
  • Species
    M. musculus (mouse)
  • Mutation
    P domain (contains residues 187-283)
  • Entrez Gene
    Calr (a.k.a. CRT, Calregulin)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • GB1 (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    CALR gene was cloned from ATCC (MGC-6209)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGB1-mCRT(P-domain) was a gift from Malini Raghavan (Addgene plasmid # 83519 ; http://n2t.net/addgene:83519 ; RRID:Addgene_83519)

  • For your References section:

    The C-Terminal Acidic Region of Calreticulin Mediates Phosphatidylserine Binding and Apoptotic Cell Phagocytosis. Wijeyesakere SJ, Bedi SK, Huynh D, Raghavan M. J Immunol. 2016 May 1;196(9):3896-909. doi: 10.4049/jimmunol.1502122. Epub 2016 Apr 1. 10.4049/jimmunol.1502122 PubMed 27036911
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