GST-FOXO3a TM 1-506aa
(Plasmid #8351)

• Depositing Lab: Michael Greenberg
• Publication: Brunet et al Cell 1999 Mar 19;96(6):857-68.

Backbone

• Vector backbone: pGex 4T3
• Backbone size w/o insert (bp): 4968
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FOXO3a 1-506aa
• Alt name: FKHRL1
• Alt name: forkhead family
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Mutation: T32A, S253A, S315A
• Entrez Gene: FOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: pGEX5'
• 3′ sequencing primer: pGEX-3

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that the stop codon in the vector backbone is used to terminate translation. Thus, a 10aa sequence (VDSSGRIVTD) will be expressed as part of FOXO3a.

See GST-FOXO3a 1-506aa for wild-type map and sequence.