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Addgene

pmcsg7-mCRT(deltaP)
(Plasmid #83507)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83507 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmcsg7
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CALR
  • Species
    M. musculus (mouse)
  • Mutation
    delta . Deletion of residues 187-283
  • Entrez Gene
    Calr (a.k.a. CRT, Calregulin)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    CALR gene was cloned from ATCC (MGC-6209)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmcsg7-mCRT(deltaP) was a gift from Malini Raghavan (Addgene plasmid # 83507 ; http://n2t.net/addgene:83507 ; RRID:Addgene_83507)

  • For your References section:

    Modes of calreticulin recruitment to the major histocompatibility complex class I assembly pathway. Del Cid N, Jeffery E, Rizvi SM, Stamper E, Peters LR, Brown WC, Provoda C, Raghavan M. J Biol Chem. 2010 Feb 12;285(7):4520-35. doi: 10.1074/jbc.M109.085407. Epub 2009 Dec 3. 10.1074/jbc.M109.085407 PubMed 19959473
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