pmcsg7-mCRT(WT)
(Plasmid
#83505)
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PurposeBacterial expression of Calreticulin
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83505 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepmcsg7
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCALR
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SpeciesM. musculus (mouse)
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Entrez GeneCalr (a.k.a. CRT, Calregulin)
- Promoter T7
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCALR gene was cloned from ATCC (MGC-6209)
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmcsg7-mCRT(WT) was a gift from Malini Raghavan (Addgene plasmid # 83505 ; http://n2t.net/addgene:83505 ; RRID:Addgene_83505) -
For your References section:
Modes of calreticulin recruitment to the major histocompatibility complex class I assembly pathway. Del Cid N, Jeffery E, Rizvi SM, Stamper E, Peters LR, Brown WC, Provoda C, Raghavan M. J Biol Chem. 2010 Feb 12;285(7):4520-35. doi: 10.1074/jbc.M109.085407. Epub 2009 Dec 3. 10.1074/jbc.M109.085407 PubMed 19959473