pWAP-HGF
(Plasmid
#83503)
-
PurposeGeneration of transgenic mice overexpressing the HGF under the control of the WAP gene promoter
-
Depositing Labs
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83503 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneWAP-SKII (pBluescript SKII)
-
Vector typeMouse Targeting
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameHGF
-
Alt namehepatocyte growth factor
-
SpeciesM. musculus (mouse)
-
Mutationalso contains beta-globin sequences from pUC198
-
Entrez GeneHgf (a.k.a. C230052L06Rik, HGF/SF, NK1, NK2, SF, SF/HGF)
- Promoter mouse WAP (whey acidic protein)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Bglob-intron-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pWAP-HGF was a gift from Marta Gallego & Lothar Hennighausen (Addgene plasmid # 83503 ; http://n2t.net/addgene:83503 ; RRID:Addgene_83503) -
For your References section:
Targeted expression of HGF/SF in mouse mammary epithelium leads to metastatic adenosquamous carcinomas through the activation of multiple signal transduction pathways. Gallego MI, Bierie B, Hennighausen L. Oncogene. 2003 Nov 20;22(52):8498-508. 10.1038/sj.onc.1207063 PubMed 14627990
