NPY-pHTomato
(Plasmid #83501)

• Purpose: Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.
• Depositing Lab: Sebastian Barg
• Publication: Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. doi: 10.1371/journal.pone.0127801. eCollection 2015.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NPY
• Alt name: neuropeptide-Y
• Species: H. sapiens (human)
• Mutation: none
• Entrez Gene: NPY (a.k.a. PYY4)
• Promoter: CMV
• Tag / Fusion Protein:
  • pHTomato (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pHTomato was provided from Yulong Li (School of Life Sciences, Peking University, Life Sciences Building, Mailbox 142, 5 Yi He Yuan Road, Haidian, Beijing, China, 100871) and Tsien 2012, Nature Neuroscience 15, 1047–1053.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry