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Addgene

pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry
(Plasmid #83491)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83491 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Total vector size (bp) 8072
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DN-AMPK-2A-mCherry
  • Mutation
    K45R

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry was a gift from Dong Kong (Addgene plasmid # 83491 ; http://n2t.net/addgene:83491 ; RRID:Addgene_83491)

  • For your References section:

    A Postsynaptic AMPK-->p21-Activated Kinase Pathway Drives Fasting-Induced Synaptic Plasticity in AgRP Neurons. Kong D, Dagon Y, Campbell JN, Guo Y, Yang Z, Yi X, Aryal P, Wellenstein K, Kahn BB, Sabatini BL, Lowell BB. Neuron. 2016 Jun 16. pii: S0896-6273(16)30204-5. doi: 10.1016/j.neuron.2016.05.025. 10.1016/j.neuron.2016.05.025 PubMed 27321921
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