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p2CT-His-MBP-Lbu_C2c2_WT
(Plasmid #83482)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83482 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p2CT
  • Backbone size w/o insert (bp) 5960
  • Total vector size (bp) 9437
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Lbu_C2c2_WT
  • Alt name
    LbuCas13a
  • Species
    Leptotrichia buccalis
  • Insert Size (bp)
    3477
  • GenBank ID
    GI_506250229
  • Promoter T7
  • Tag / Fusion Protein
    • His6-MBP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Codon optimized for E. Coli expression. His-MBP fusion tag can be removed with TEV protease.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2CT-His-MBP-Lbu_C2c2_WT was a gift from Jennifer Doudna (Addgene plasmid # 83482 ; http://n2t.net/addgene:83482 ; RRID:Addgene_83482)

  • For your References section:

    Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian R, Doudna JA. Nature. 2016 Sep 26. doi: 10.1038/nature19802. 10.1038/nature19802 PubMed 27669025
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