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PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83477 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSII426
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Vector typeYeast Expression
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Selectable markersnourseothricin (nat)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB dam-/dcm-
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Growth instructionsGrowth in dam- E.coli cells is preferred
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNatMx3
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pRS-marker
- 3′ sequencing primer F1ori-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene plasmid #67638 pML104, deposited by John Wyrick
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is similar to pML104 except that the URA3 marker has been switched for NatMx3 marker using Gibson Assembly. Cloning of guide sequence using BclI-SwaII restriction sites requires growth in dam- E. coli cells.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pML104-NatMx3 was a gift from Patricia Wittkopp (Addgene plasmid # 83477 ; http://n2t.net/addgene:83477 ; RRID:Addgene_83477)