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PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83475 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSII426
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Vector typeYeast Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB dam-/dcm-
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Growth instructionsGrowth in dam- E.coli cells is preferred
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHphMX4
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pRS-marker
- 3′ sequencing primer F1ori-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene plasmid #67638 pML104, deposited by John Wyrick
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is similar to pML104 except that the URA3 marker has been switched for hphMX4 marker using Gibson Assembly. Cloning of guide sequence using BclI-SwaII restriction sites requires growth in dam- E. coli cells.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pML104-HygMx4 was a gift from Patricia Wittkopp (Addgene plasmid # 83475 ; http://n2t.net/addgene:83475 ; RRID:Addgene_83475)
