Tet-myr-CSK-GFP
(Plasmid
#83470)
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PurposeLentiviral vector for doxycycline-inducible expression of membrane targeted-CSK in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83470 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneTRIPZ-non-silencing shRNA control
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Backbone manufacturerGE-Healthcare
- Backbone size w/o insert (bp) 12872
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin, Zeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCSK
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Alt namec-src tyrosine kinase
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SpeciesH. sapiens (human)
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Insert Size (bp)2130
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GenBank ID1445
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Entrez GeneCSK
- Promoter doxycycline-inducible promoter
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer LNCX
- 3′ sequencing primer hUBCpro-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Tet-myr-CSK-GFP was a gift from Barry Gumbiner (Addgene plasmid # 83470 ; http://n2t.net/addgene:83470 ; RRID:Addgene_83470) -
For your References section:
Adhesion to fibronectin regulates Hippo signaling via the FAK-Src-PI3K pathway. Kim NG, Gumbiner BM. J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. Epub 2015 Jul 27. 10.1083/jcb.201501025 PubMed 26216901
