Tet-CA-Src-GFP
(Plasmid #83469)

• Purpose: Lentiviral vector for doxycycline-inducible expression of constitutively active c-Src in mammalian cells
• Depositing Lab: Barry Gumbiner
• Publication: Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. Epub 2015 Jul 27.

Backbone

• Vector backbone: TRIPZ-non-silencing shRNA control
• Backbone manufacturer: GE-Healthcare
• Backbone size w/o insert (bp): 12803
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin, Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Src
• Species: G. gallus (chicken)
• Insert Size (bp): 2349
• Mutation: Y527F (constitutively active)
• GenBank ID: 396442
• Entrez Gene: SRC (a.k.a. PP60C-SCR, SDR, c-src)
• Promoter: doxycycline-inducible promoter
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: hUBCpro-F

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: pEGFP N1-Y527F Src plasmid from Drs Emma Sandilands & Margaret C Frame (The Beatson Institute for Cancer Research, Glasgow UK) was used to generate this plasmid.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry