pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4303)
(Plasmid
#83465)
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PurposeCytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to amino acids 4078 to 4303 of PC1 (98)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83465 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA4/TO/2xMyc/His
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePolycystin-1
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SpeciesH. sapiens (human)
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MutationAmino-acids 4078-4303
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Entrez GenePKD1 (a.k.a. PBP, PC1, Pc-1, TRPP1)
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Tag
/ Fusion Protein
- CD16.7 (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4303) was a gift from Thomas Weimbs (Addgene plasmid # 83465 ; http://n2t.net/addgene:83465 ; RRID:Addgene_83465) -
For your References section:
Regulation of Polycystin-1 Function by Calmodulin Binding. Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T. PLoS One. 2016 Aug 25;11(8):e0161525. doi: 10.1371/journal.pone.0161525. eCollection 2016. PONE-D-16-13986 [pii] PubMed 27560828
