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Addgene

pcDNA4/TO h-PKD1 FLS L4137P-GST
(Plasmid #83455)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83455 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/TO/2xMyc/His
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Polycystin-1
  • Species
    H. sapiens (human)
  • Mutation
    Amino-acids 4107-4303, L4137P
  • Entrez Gene
    PKD1 (a.k.a. PBP, PC1, Pc-1, TRPP1)
  • Tag / Fusion Protein
    • GST (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4/TO h-PKD1 FLS L4137P-GST was a gift from Thomas Weimbs (Addgene plasmid # 83455 ; http://n2t.net/addgene:83455 ; RRID:Addgene_83455)

  • For your References section:

    Regulation of Polycystin-1 Function by Calmodulin Binding. Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T. PLoS One. 2016 Aug 25;11(8):e0161525. doi: 10.1371/journal.pone.0161525. eCollection 2016. PONE-D-16-13986 [pii] PubMed 27560828
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