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pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His
(Plasmid #83453)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83453 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/TO/2xMyc/His
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    polycystin-1
  • Alt name
    PKD1
  • Species
    M. musculus (mouse)
  • Mutation
    S4144D
  • GenBank ID
  • Entrez Gene
    Pkd1 (a.k.a. PC1, mFLJ00285)
  • Tags / Fusion Proteins
    • 3xFlag (N terminal on insert)
    • 2xMyc (C terminal on insert)
    • 6xHis (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His was a gift from Thomas Weimbs (Addgene plasmid # 83453 ; http://n2t.net/addgene:83453 ; RRID:Addgene_83453)

  • For your References section:

    Regulation of Polycystin-1 Function by Calmodulin Binding. Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T. PLoS One. 2016 Aug 25;11(8):e0161525. doi: 10.1371/journal.pone.0161525. eCollection 2016. PONE-D-16-13986 [pii] PubMed 27560828
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