pcDNA4/TO 3xFlag-m-PKD1-2xMyc/His
(Plasmid #83452)

• Purpose: Full-length murine PC1 for Eukaryotic Expression (982)
• Depositing Lab: Thomas Weimbs
• Publication: Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. doi: 10.1371/journal.pone.0161525. eCollection 2016.

Backbone

• Vector backbone: pcDNA4/TO/2xMyc/His
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: polycystin-1
• Alt name: PKD1
• Species: M. musculus (mouse)
• GenBank ID:
• Entrez Gene: Pkd1 (a.k.a. PC1, mFLJ00285)
• Tags / Fusion Proteins:
  • 3xFlag (N terminal on insert)
  • 2xMyc (C terminal on backbone)
  • 6xHis (C terminal on backbone)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry