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Addgene

hFLT1
(Plasmid #83435)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83435 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV6 Entry
  • Backbone manufacturer
    Origene
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human VEGFR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4283
  • Mutation
    HA-tag was inserted 30 AA downstream of the FLT1 start codon
  • Entrez Gene
    FLT1 (a.k.a. FLT, FLT-1, VEGFR-1, VEGFR1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Myc (C terminal on backbone)
    • Flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-PA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hFLT1 was a gift from Christie Thomas (Addgene plasmid # 83435 ; http://n2t.net/addgene:83435 ; RRID:Addgene_83435)

  • For your References section:

    Protein kinase C regulates FLT1 abundance and stimulates its cleavage in vascular endothelial cells with the release of a soluble PlGF/VEGF antagonist. Raikwar NS, Liu KZ, Thomas CP. Exp Cell Res. 2013 Oct 15;319(17):2578-87. doi: 10.1016/j.yexcr.2013.07.005. Epub 2013 Jul 30. 10.1016/j.yexcr.2013.07.005 PubMed 23911939
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