hNedd4-2
(Plasmid #83433)

• Purpose: full length Nedd4L with His Xpress tag
• Depositing Lab: Christie Thomas
• Publication: Itani et al Am J Physiol Renal Physiol. 2005 Aug;289(2):F334-46. Epub 2005 Apr 5.

Backbone

• Vector backbone: pcDNA4 HisMax
• Backbone manufacturer: thermofisher
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human Nedd4L with C2 domain
• Species: H. sapiens (human)
• Insert Size (bp): 2889
• GenBank ID: NM_001144967.2 NP_001138439.1
• Entrez Gene: NEDD4L (a.k.a. NEDD4-2, NEDD4.2, PVNH7, RSP5, hNEDD4-2)
• Promoter: CMV
• Tags / Fusion Proteins:
  • His Tag (6x) (N terminal on backbone)
  • Xpress Epitope Tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry