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Addgene

h-alpha-ENaC-2
(Plasmid #83431)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83431 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    thermofisher
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human alpha ENaC isoform 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2190
  • Entrez Gene
    SCNN1A (a.k.a. BESC2, ENaCa, ENaCalpha, LIDLS3, PHA1B1, SCNEA, SCNN1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    h-alpha-ENaC-2 was a gift from Christie Thomas (Addgene plasmid # 83431 ; http://n2t.net/addgene:83431 ; RRID:Addgene_83431)

  • For your References section:

    5' heterogeneity in epithelial sodium channel alpha-subunit mRNA leads to distinct NH2-terminal variant proteins. Thomas CP, Auerbach S, Stokes JB, Volk KA. Am J Physiol. 1998 May;274(5 Pt 1):C1312-23. PubMed 9612219
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