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Addgene

h-beta-ENaC
(Plasmid #83429)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83429 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    thermofisher
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human beta ENaC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2075
  • Entrez Gene
    SCNN1B (a.k.a. BESC1, ENaCb, ENaCbeta, LIDLS1, PHA1B2, SCNEB, beta-ENaC, beta-NaCH)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    h-beta-ENaC was a gift from Christie Thomas (Addgene plasmid # 83429 ; http://n2t.net/addgene:83429 ; RRID:Addgene_83429)

  • For your References section:

    Nedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the epithelial sodium channel. Raikwar NS, Thomas CP. Am J Physiol Renal Physiol. 2008 May;294(5):F1157-65. doi: 10.1152/ajprenal.00339.2007. Epub 2008 Mar 5. 10.1152/ajprenal.00339.2007 PubMed 18322022
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