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hum alpha ENAC promoter a25-a23
(Plasmid #83427)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83427 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3basic
  • Backbone manufacturer
    Promega
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A
  • Alt name
    aENaC a25a23
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1443
  • Entrez Gene
    SCNN1A (a.k.a. BESC2, ENaCa, ENaCalpha, LIDLS3, PHA1B1, SCNEA, SCNN1)
  • Promoter alpha ENAC
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sac1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNRev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hum alpha ENAC promoter a25-a23 was a gift from Christie Thomas (Addgene plasmid # 83427 ; http://n2t.net/addgene:83427 ; RRID:Addgene_83427)

  • For your References section:

    Glucocorticoid induction of epithelial sodium channel expression in lung and renal epithelia occurs via trans-activation of a hormone response element in the 5'-flanking region of the human epithelial sodium channel alpha subunit gene. Sayegh R, Auerbach SD, Li X, Loftus RW, Husted RF, Stokes JB, Thomas CP. J Biol Chem. 1999 Apr 30;274(18):12431-7. PubMed 10212217
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