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hum sgk promoter F4R4
(Plasmid #83426)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83426 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3basic
  • Backbone manufacturer
    Promega
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR
  • Alt name
    Sgk1F4R4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3259
  • Entrez Gene
    SGK1 (a.k.a. SGK)
  • Promoter sgk
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNRev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hum sgk promoter F4R4 was a gift from Christie Thomas (Addgene plasmid # 83426 ; http://n2t.net/addgene:83426 ; RRID:Addgene_83426)

  • For your References section:

    Glucocorticoids stimulate human sgk1 gene expression by activation of a GRE in its 5'-flanking region. Itani OA, Liu KZ, Cornish KL, Campbell JR, Thomas CP. Am J Physiol Endocrinol Metab. 2002 Nov;283(5):E971-9. 10.1152/ajpendo.00021.2002 PubMed 12376324
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