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Addgene

dsRedm-Rab21
(Plasmid #83425)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83425 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDsRed-monomer-C1
  • Backbone size w/o insert (bp) 4639
  • Total vector size (bp) 5316
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rab21
  • Alt name
    Mus musculus RAB21, member RAS oncogene family (Rab21)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    677
  • GenBank ID
    NM_024454.1
  • Entrez Gene
    Rab21 (a.k.a. 9630024B22)
  • Promoter CMV
  • Tag / Fusion Protein
    • dsRed-monomer (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer DsRed-Monomer-C sequencing primer: AGCTGGACATCACCAACCACAACG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dsRedm-Rab21 was a gift from Johanna Ivaska (Addgene plasmid # 83425 ; http://n2t.net/addgene:83425 ; RRID:Addgene_83425)

  • For your References section:

    Integrin trafficking regulated by Rab21 is necessary for cytokinesis. Pellinen T, Tuomi S, Arjonen A, Wolf M, Edgren H, Meyer H, Grosse R, Kitzing T, Rantala JK, Kallioniemi O, Fassler R, Kallio M, Ivaska J. Dev Cell. 2008 Sep;15(3):371-85. doi: 10.1016/j.devcel.2008.08.001. 10.1016/j.devcel.2008.08.001 PubMed 18804435
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