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pCDNA5_FKBP25-V5-APEX2
(Plasmid #83418)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83418 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5
  • Backbone size w/o insert (bp) 5082
  • Total vector size (bp) 6609
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Peptidyl-prolyl cis-trans isomerase FKBP3
  • Alt name
    FKBP25
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    672
  • GenBank ID
    NM_002013.3. NM_002013.3.
  • Entrez Gene
    FKBP3 (a.k.a. FKBP-25, FKBP-3, FKBP25, PPIase)
  • Promoter CMV-F
  • Tags / Fusion Proteins
    • V5 (C terminal on insert)
    • APEX2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA5_FKBP25-V5-APEX2 was a gift from Hyun-Woo Rhee (Addgene plasmid # 83418 ; http://n2t.net/addgene:83418 ; RRID:Addgene_83418)

  • For your References section:

    Proximity-Directed Labeling Reveals a New Rapamycin-Induced Heterodimer of FKBP25 and FRB in Live Cells. Lee SY, Lee H, Lee HK, Lee SW, Ha SC, Kwon T, Seo JK, Lee C, Rhee HW. ACS Cent Sci. 2016 Aug 24;2(8):506-16. doi: 10.1021/acscentsci.6b00137. Epub 2016 Aug 12. 10.1021/acscentsci.6b00137 PubMed 27610411
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