pCDNA5_FRB-pBirA-HA
(Plasmid #83415)

• Purpose: proximity-directed biotin labeling on the FRB domain of mTOR in mammalian cell
• Depositing Lab: Hyun-Woo Rhee
• Publication: Lee et al ACS Cent Sci. 2016 Aug 24;2(8):506-16. doi: 10.1021/acscentsci.6b00137. Epub 2016 Aug 12.

Backbone

• Vector backbone: pcDNA5
• Backbone size w/o insert (bp): 5082
• Total vector size (bp): 6384
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FRB-pBirA-HA
• Alt name: FKBP12-rapamycin binding domain
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1302
• GenBank ID: NM_004958.3.
• Entrez Gene: MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
• Promoter: CMV-F
• Tags / Fusion Proteins:
  • pBirA (C terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry