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pCDNA3_UnaG-Flag-Sec61B
(Plasmid #83413)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83413 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5378
  • Total vector size (bp) 6120
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UnaG-Sec61B
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    742
  • GenBank ID
    NM_006808.2. NM_006808.2.
  • Entrez Gene
    SEC61B

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer tagtaggaattcatgcctggtccgacccc
  • 3′ sequencing primer tagtagctcgagTCAcgaacgagtgtacttgcccca
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3_UnaG-Flag-Sec61B was a gift from Hyun-Woo Rhee (Addgene plasmid # 83413 ; http://n2t.net/addgene:83413 ; RRID:Addgene_83413)

  • For your References section:

    In Cellulo Mapping of Subcellular Localized Bilirubin. Park JS, Nam E, Lee HK, Lim MH, Rhee HW. ACS Chem Biol. 2016 May 27. 10.1021/acschembio.6b00017 PubMed 27232847
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