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pCDNA3_V5-APEX2-Sec61B
(Plasmid #83412)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83412 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5382
  • Total vector size (bp) 6477
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    V5-APEX2-Sec61B
  • Alt name
    Protein transport protein Sec61 subunit beta
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1095
  • GenBank ID
    NM_006808.2.
  • Entrez Gene
    SEC61B
  • Promoter CMV-F
  • Tags / Fusion Proteins
    • V5 (N terminal on insert)
    • APEX2 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3_V5-APEX2-Sec61B was a gift from Hyun-Woo Rhee (Addgene plasmid # 83412 ; http://n2t.net/addgene:83412 ; RRID:Addgene_83412)

  • For your References section:

    APEX Fingerprinting Reveals the Subcellular Localization of Proteins of Interest. Lee SY, Kang MG, Park JS, Lee G, Ting AY, Rhee HW. Cell Rep. 2016 May 11. pii: S2211-1247(16)30508-3. doi: 10.1016/j.celrep.2016.04.064. 10.1016/j.celrep.2016.04.064 PubMed 27184847
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