pRS406-CMV-lacI-3FLAG
(Plasmid
#83410)
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PurposeExpression of lacI in yeast
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83410 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS406
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namelacI repressor
- Promoter CMV
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Tags
/ Fusion Proteins
- NLS (C terminal on insert)
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
We cut the plasmid by digestion with BstBI within URA3 and integrate it at the ura3 locus.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS406-CMV-lacI-3FLAG was a gift from Toshi Tsukiyama (Addgene plasmid # 83410 ; http://n2t.net/addgene:83410 ; RRID:Addgene_83410) -
For your References section:
Dynamic changes in histone acetylation regulate origins of DNA replication. Unnikrishnan A, Gafken PR, Tsukiyama T. Nat Struct Mol Biol. 2010 Apr;17(4):430-7. doi: 10.1038/nsmb.1780. Epub 2010 Mar 14. 10.1038/nsmb.1780 PubMed 20228802
Map uploaded by the depositor.
