pcdna3.1 hciap2mut
(Plasmid #8339)

• Depositing Lab: Jon Ashwell
• Publication: Li et al Nature 2002 Mar 21;416(6878):345-7.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ciap2mut
• Alt name: cIAP2
• Species: H. sapiens (human)
• Insert Size (bp): 1800
• Mutation: H574A
• Entrez Gene: BIRC3 (a.k.a. AIP1, API2, CIAP2, HAIP1, HIAP1, IAP-1, MALT2, MIHC, RNF49, c-IAP2)
• Tag / Fusion Protein:
  • myc

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a N100D change in the amino acid sequence, according to the author this mutation does not affect protein function.