Talin-CS
(Plasmid #83377)

• Purpose: Control FRET probe for measuring tension on Talin molecule
• Depositing Lab: Martin Schwartz
• Publication: Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. doi: 10.1083/jcb.201510012.

Backbone

• Vector backbone: pLPCX
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Talin1
• Species: M. musculus (mouse)
• Insert Size (bp): 9198
• Mutation: Tension sensor module is inserted at C-terminal of talin 1
• GenBank ID: 21894
• Entrez Gene: Tln1 (a.k.a. Tln)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP-F40-tagRFP (tension sensor module) (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: LNCX-3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

XhoI RE site in the vector backbone is disrupted. Insert also contains ClaI site.
F40 refers to the 40-aa peptide (GPGGA)8 derived from the elastic spider silk protein flagelliform