pCMV-Myc-GFP-Vpr-RR
(Plasmid
#83374)
-
PurposeExpression of Myc tagged GFP-Vpr fusion protein
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 83374 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3.1/zeo
-
Backbone manufacturer...
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMyc-GFP-Vpr
-
SpeciesSynthetic
-
Insert Size (bp)1020
- Promoter CMV
-
Tags
/ Fusion Proteins
- Myc (N terminal on insert)
- Myc-GFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV-Myc-GFP-Vpr-RR was a gift from Wesley Sundquist (Addgene plasmid # 83374 ; http://n2t.net/addgene:83374 ; RRID:Addgene_83374) -
For your References section:
Designed proteins induce the formation of nanocage-containing extracellular vesicles. Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King NP, Sundquist WI. Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub 2016 Nov 30. 10.1038/nature20607 PubMed 27919066