pADtet-His6-MscL
(Plasmid #83373)

• Purpose: Expresses wild type MscL in mammalian cells
• Depositing Lab: Allen Liu
• Publication: Heureaux et al Cell Mol Bioeng. 2014 Sep;7(3):307-319.

Backbone

• Vector backbone: pADtet
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Mechanosensitive channel of large conductance
• Alt name: MscL
• Species: E. coli
• Insert Size (bp): 408
• GenBank ID: NC_000913.3
• Entrez Gene: mscL (a.k.a. b3291, ECK3277, yhdC)
• Promoter: CMV
• Tag / Fusion Protein:
  • His6 tag (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMVmin
• 3′ sequencing primer: SV40 polyA

Resource Information

• Supplemental Documents:
  • Forward sequencing of MscL insert
  • Reverse sequencing of MscL insert
• A portion of this plasmid was derived from a plasmid made by: Dr. Boris Martinac from Victor Chang Cardiac Research Institute in Australia

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry