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pET28b(+)-SPN+IFS
(Plasmid #83372)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83372 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5368
  • Total vector size (bp) 7099
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    SPN
  • Alt name
    Nicotine adenine dinucleotide glycohydrolase
  • Alt name
    Nga
  • Species
    Streptococcus pyogenes serotype M3
  • Insert Size (bp)
    1245
  • GenBank ID
    AAM78735.1
  • Promoter T7
  • Tag / Fusion Protein
    • Hexahistidine tag (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site None (not destroyed)
  • 5′ sequencing primer T7 promoter
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    IFS
  • Species
    Streptococcus pyogenes serotype M3
  • Insert Size (bp)
    486
  • GenBank ID
    AAM78736.1
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer None
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b(+)-SPN+IFS was a gift from SeWon Suh (Addgene plasmid # 83372 ; http://n2t.net/addgene:83372 ; RRID:Addgene_83372)

  • For your References section:

    High-resolution crystal structure of Streptococcus pyogenes beta-NAD(+) glycohydrolase in complex with its endogenous inhibitor IFS reveals a highly water-rich interface. Yoon JY, An DR, Yoon HJ, Kim HS, Lee SJ, Im HN, Jang JY, Suh SW. J Synchrotron Radiat. 2013 Nov;20(Pt 6):962-7. doi: 10.1107/S0909049513020803. Epub 2013 Sep 29. 10.1107/S0909049513020803 PubMed 24121349
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