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PurposeFor tagging mitochondria with HA epitopes (HA-MITO construct)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 83356 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXs rtv-016
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Backbone manufacturerCell BioLabs
- Backbone size w/o insert (bp) 5600
- Total vector size (bp) 6600
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Modifications to backboneAll restriction sites are intact from original vector rtv-016: cloned insert into MCS with XhoI and NotI
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Vector typeMammalian Expression, Retroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert name3XHA-EGFP-OMP25
Resource Information
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Supplemental Documents
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs-3XHA-EGFP-OMP25 was a gift from David Sabatini (Addgene plasmid # 83356 ; http://n2t.net/addgene:83356 ; RRID:Addgene_83356) -
For your References section:
Absolute Quantification of Matrix Metabolites Reveals the Dynamics of Mitochondrial Metabolism. Chen WW, Freinkman E, Wang T, Birsoy K, Sabatini DM. Cell. 2016 Aug 25;166(5):1324-1337.e11. doi: 10.1016/j.cell.2016.07.040. 10.1016/j.cell.2016.07.040 PubMed 27565352