Pentaprobe PP5
(Plasmid
#83330)
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PurposePentaprobe for assesing the RNA binding potential of a protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83330 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression ; RNA transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePP5
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SpeciesSynthetic
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Insert Size (bp)100
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Pentaprobe PP5 was a gift from Joel Mackay (Addgene plasmid # 83330 ; http://n2t.net/addgene:83330 ; RRID:Addgene_83330) -
For your References section:
A rapid method for assessing the RNA-binding potential of a protein. Bendak K, Loughlin FE, Cheung V, O'Connell MR, Crossley M, Mackay JP. Nucleic Acids Res. 2012 Aug;40(14):e105. doi: 10.1093/nar/gks285. Epub 2012 Apr 3. 10.1093/nar/gks285 PubMed 22492509
