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Addgene

pSucCD2
(Plasmid #83324)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83324 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET42b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5930
  • Total vector size (bp) 7930
  • Modifications to backbone
    C-terminal His-tag NOT expressed
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing laboratory recommends strain C41(DE3) for protein expression
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sucCD
  • Species
    E. coli
  • Insert Size (bp)
    2000

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSucCD2 was a gift from Robert Hausinger (Addgene plasmid # 83324 ; http://n2t.net/addgene:83324 ; RRID:Addgene_83324)
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