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Addgene

289 RFP/CFP
(Plasmid #83321)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83321 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    peCFP N1
  • Total vector size (bp) 6179
  • Vector type
    Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RFP +CFP joining substrate
  • Species
    Synthetic; multiple

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site multiple (unknown if destroyed)
  • 3′ cloning site multiple (unknown if destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    289 RFP/CFP was a gift from Katheryn Meek (Addgene plasmid # 83321 ; http://n2t.net/addgene:83321 ; RRID:Addgene_83321)

  • For your References section:

    Restoration of ATM Expression in DNA-PKcs-Deficient Cells Inhibits Signal End Joining. Neal JA, Xu Y, Abe M, Hendrickson E, Meek K. J Immunol. 2016 Apr 1;196(7):3032-42. doi: 10.4049/jimmunol.1501654. Epub 2016 Feb 26. 10.4049/jimmunol.1501654 PubMed 26921311
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