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Purposehuman DNA-PKcs/ABCDE>ala
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 83318 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcmv6
- Backbone size w/o insert (bp) 4665
- Total vector size (bp) 17065
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl2
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Growth instructionssee attached propagation instructions. This plasmid is prone to recombination. Transformed E. coli is extremely slow growing; please pick smallest colonies for a test prep, then check for appropriate EcoR1 restriction pattern. Usually, after transformation there will be a population of larger colonies… these are clones that have deletions.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman DNA-PKcs
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SpeciesH. sapiens (human)
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Insert Size (bp)12387
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Mutation6 phosphorylation sites in the ABCDE cluster substituted to alanine
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Entrez GenePRKDC (a.k.a. DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, IMD26, XRCC7, p350)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DNA-PKcs ABCDE>A was a gift from Katheryn Meek (Addgene plasmid # 83318 ; http://n2t.net/addgene:83318 ; RRID:Addgene_83318) -
For your References section:
Autophosphorylation of DNA-dependent protein kinase regulates DNA end processing and may also alter double-strand break repair pathway choice. Cui X, Yu Y, Gupta S, Cho YM, Lees-Miller SP, Meek K. Mol Cell Biol. 2005 Dec;25(24):10842-52. 10.1128/MCB.25.24.10842-10852.2005 PubMed 16314509