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pQE30-roGFP1-iE
(Plasmid #83312)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83312 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE30
  • Backbone manufacturer
    Qiagen
  • Backbone size w/o insert (bp) 3400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    roGFP1-iE
  • Alt name
    ro1/iE
  • Species
    jellyfish
  • Insert Size (bp)
    780
  • Mutation
    ro1(C48S/S147C/Q204C) plus X insertion, iE between 147C and H148S/F64L/F99S/V163A/I167T (ro1/iE is missing the M153T mutation which is reported to be there in the paper)
  • Promoter T5
  • Tag / Fusion Protein
    • 6xHIS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pQE-F2
  • 3′ sequencing primer T0term-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE30-roGFP1-iE was a gift from S. James Remington (Addgene plasmid # 83312 ; http://n2t.net/addgene:83312 ; RRID:Addgene_83312)

  • For your References section:

    Development of a family of redox-sensitive green fluorescent protein indicators for use in relatively oxidizing subcellular environments. Lohman JR, Remington SJ. Biochemistry. 2008 Aug 19;47(33):8678-88. doi: 10.1021/bi800498g. Epub 2008 Jul 25. 10.1021/bi800498g PubMed 18652491
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