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Addgene

pRSETB-roGFP1-R10
(Plasmid #83310)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83310 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSETB
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    roGFP1-R10
  • Alt name
    ro1/10
  • Species
    jellyfish
  • Insert Size (bp)
    714
  • Mutation
    C48S/Q80R/S147C/Q204C/F223R/S202K/K41D
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHIS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSETB-roGFP1-R10 was a gift from S. James Remington (Addgene plasmid # 83310 ; http://n2t.net/addgene:83310 ; RRID:Addgene_83310)

  • For your References section:

    Re-engineering redox-sensitive green fluorescent protein for improved response rate. Cannon MB, Remington SJ. Protein Sci. 2006 Jan;15(1):45-57. Epub 2005 Dec 1. 10.1110/ps.051734306 PubMed 16322566
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