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csd2_121-308,Y197F/pET28b(+)
(Plasmid #83299)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83299 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hp1544
  • Alt name
    ToxR-activated gene (TagE)
  • Species
    H. pylori 26695 strain
  • Insert Size (bp)
    564
  • Mutation
    deleted amino acid 1-120, and changed Tyrosine 197 to Phenylalanine
  • GenBank ID
    AAD08583.1
  • Tags / Fusion Proteins
    • His tag (N terminal on backbone)
    • His tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    csd2_121-308,Y197F/pET28b(+) was a gift from SeWon Suh (Addgene plasmid # 83299 ; http://n2t.net/addgene:83299 ; RRID:Addgene_83299)

  • For your References section:

    Structural Basis of the Heterodimer Formation between Cell Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori. An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, Mobashery S, Kim SJ, Suh SW. PLoS One. 2016 Oct 6;11(10):e0164243. doi: 10.1371/journal.pone.0164243. eCollection 2016. 10.1371/journal.pone.0164243 PubMed 27711177
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