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Addgene

csd4_22-438/pET28b(+)
(Plasmid #83295)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83295 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hp1075
  • Alt name
    Csd4
  • Species
    H. pylori 26695 strain
  • Insert Size (bp)
    1251
  • Mutation
    deleted amino acid 1-21
  • GenBank ID
    AAD08118.1
  • Tag / Fusion Protein
    • His tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    csd4_22-438/pET28b(+) was a gift from SeWon Suh (Addgene plasmid # 83295 ; http://n2t.net/addgene:83295 ; RRID:Addgene_83295)

  • For your References section:

    Structural basis for the recognition of muramyltripeptide by Helicobacter pylori Csd4, a D,L-carboxypeptidase controlling the helical cell shape. Kim HS, Kim J, Im HN, An DR, Lee M, Hesek D, Mobashery S, Kim JY, Cho K, Yoon HJ, Han BW, Lee BI, Suh SW. Acta Crystallogr D Biol Crystallogr. 2014 Nov;70(Pt 11):2800-12. doi: 10.1107/S1399004714018732. Epub 2014 Oct 16. 10.1107/S1399004714018732 PubMed 25372672
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