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Addgene

pEN_TT miRc2 3xFLAG
(Plasmid #83274)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83274 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEN_TTmiRc2
  • Backbone manufacturer
    Addgene #25752
  • Backbone size (bp) 4496
  • Modifications to backbone
    3xFLAG cloned in before the restriction sites
  • Vector type
    entry vector for gateway cloning
  • Promoter TRE tight
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    DB3.1 (ccdB survival)
  • Copy number
    Unknown

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN_TT miRc2 3xFLAG was a gift from Kevin Janes (Addgene plasmid # 83274 ; http://n2t.net/addgene:83274 ; RRID:Addgene_83274)

  • For your References section:

    Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA. Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 10.1016/j.devcel.2017.10.027. 10.1016/j.devcel.2017.10.027 PubMed 29161592
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