pSLIK TT 3xFLAG SMAD4 neo
(Plasmid
#83273)
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PurposeLentiviral vector for inducible expression of SMAD4 with 3xFLAG N-terminal tag
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83273 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSLIK-neo
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Backbone manufacturerAddgene #25735
- Total vector size (bp) 13286
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSMAD4
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Alt nameDPC4
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Alt nameMADH4
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Alt nameSMAD family member 4
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SpeciesH. sapiens (human)
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Insert Size (bp)1659
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GenBank IDNM_005359
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Entrez GeneSMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hUBCpro-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Lentiviral mammalian expression plasmid for inducible expression of 3xFLAG SMAD4
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLIK TT 3xFLAG SMAD4 neo was a gift from Kevin Janes (Addgene plasmid # 83273 ; http://n2t.net/addgene:83273 ; RRID:Addgene_83273) -
For your References section:
Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA. Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 10.1016/j.devcel.2017.10.027. 10.1016/j.devcel.2017.10.027 PubMed 29161592