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Addgene

pcDNA3-TRPgamma
(Plasmid #83271)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83271 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TRPgamma
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    Trpgamma (a.k.a. Dmel_CG5996, CG5996, DmTRPgamma, Dmel\CG5996, TRPgamma, Trp-gamma, dTRPg, dTRPgamma, trp-gamma, trpgamma)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional Reference: Akitake et al., Nature Communcations 2015 Jun 1;6:7288. https://www.ncbi.nlm.nih.gov/pubmed/26028119

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-TRPgamma was a gift from Craig Montell (Addgene plasmid # 83271 ; http://n2t.net/addgene:83271 ; RRID:Addgene_83271)

  • For your References section:

    TRPgamma, a drosophila TRP-related subunit, forms a regulated cation channel with TRPL. Xu XZ, Chien F, Butler A, Salkoff L, Montell C. Neuron. 2000 Jun;26(3):647-57. S0896-6273(00)81201-5 [pii] PubMed 10896160
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