pAD-CMV-STAT5B-DN-CMV-GFP
(Plasmid
#83262)
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PurposeAdenoviral expression of dominant negative STAT5B with co-expression of GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 83262 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAD/CMV/V5-DEST
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Backbone manufacturerInvitrogen
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Vector typeAdenoviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameStat5b
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Alt nameSignal transducer and activator of transcription 5B
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SpeciesM. musculus (mouse)
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Mutationdominant negative (Y699F)
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Entrez GeneStat5b
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameeGFP
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Speciesjellyfish
- Promoter CMV
Cloning Information for Gene/Insert 2
- Cloning method Gateway Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAD-CMV-STAT5B-DN-CMV-GFP was a gift from Andrew Brooks (Addgene plasmid # 83262 ; http://n2t.net/addgene:83262 ; RRID:Addgene_83262) -
For your References section:
STAT5 Activation in the Dermal Papilla Is Important for Hair Follicle Growth Phase Induction. Legrand JM, Roy E, Ellis JJ, Francois M, Brooks AJ, Khosrotehrani K. J Invest Dermatol. 2016 Sep;136(9):1781-91. doi: 10.1016/j.jid.2016.04.014. Epub 2016 Apr 27. 10.1016/j.jid.2016.04.014 PubMed 27131881