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Addgene

Ir25a_GAL80
(Plasmid #83259)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBPGAL80w
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ir25a enhancer
  • Species
    D. melanogaster (fly)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: The Ir25a promoter was amplified from fly strain CS while the provided promoter sequence is from fly strain w1118. Therefore, there are some base changes between the provided full plasmid sequence and the actual plasmid (as seen in the Addgene quality control sequencing results) in the promoter region. The depositing laboratory is aware of these base changes; they do not affect plasmid fuction.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ir25a_GAL80 was a gift from Leslie Vosshall (Addgene plasmid # 83259 ; http://n2t.net/addgene:83259 ; RRID:Addgene_83259)

  • For your References section:

    A Taste Circuit that Regulates Ingestion by Integrating Food and Hunger Signals. Yapici N, Cohn R, Schusterreiter C, Ruta V, Vosshall LB. Cell. 2016 Apr 21;165(3):715-29. doi: 10.1016/j.cell.2016.02.061. Epub 2016 Mar 31. 10.1016/j.cell.2016.02.061 PubMed 27040496
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