pSP64R1 SHP2 WT
(Plasmid
#8325)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 8325 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSP64R1
- Backbone size w/o insert (bp) 3500
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Vector typeXenopus expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSHP2
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Alt nameSH-PTP2
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Alt namePTPN11
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SpeciesH. sapiens (human)
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Insert Size (bp)2276
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GenBank IDL03535
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Entrez GenePTPN11 (a.k.a. BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSP64R1 SHP2 WT was a gift from Ben Neel (Addgene plasmid # 8325 ; http://n2t.net/addgene:8325 ; RRID:Addgene_8325) -
For your References section:
Activated mutants of SHP-2 preferentially induce elongation of Xenopus animal caps. O'Reilly AM, Pluskey S, Shoelson SE, Neel BG. Mol Cell Biol 2000 Jan;20(1):299-311. 10.1128/MCB.20.1.299-311.2000 PubMed 10594032